The Melbourne Protein Characterisation (MPC), located within the Bio21 Institute, is a diverse facility for protein production and investigating protein quality control, molecular interactions and structural analysis. The Platform incorporates part of the Australian Cancer Research Foundation)(ACRF) Facility for Innovative Cancer Drug Discovery and forms part of the drug discovery pipeline that connects the Bio21 platforms together. MPC has 4 facilities which are Protein Production, Protein Interaction, X-ray Diffraction and Bio21-WEHI Crystallisation Facility.
The MPC capabilities include:
Protein Production & Peptide Synthesis
Insect and mammalian cells tissue culture
Microwave peptide synthesizer
Protein Interaction
Study of molecular binding and interactions
Analysis of oligomerisation and association states
Determination of protein size, shape and secondary structure
Study of heterogeneity and aggregation
Analysis of fluorescence and UV absorbance
Detection of small molecule binding
X-ray Diffraction
Analysis of structure for small molecules and proteins
Crystallisation
Screening of initial crystallisation conditions
Lipid cubic phase crystallisation
Optimisation of Hit crystallisation conditions
Crystal plate imaging
Platform is open-access, offering services to both academic and industry users, including training by platform technology specialists and fee-for-service options.
Enable accurate determination of the native molecular weight(s) of a sample, estimation of the binding affinity (i.e. KD) of self- and hetero-associating systems and stoichiometry.
Experiments generally require 100μl of sample and 120μl of reference (i.e. buffer blank)
Experiments are conducted at multiple low angular velocities (e.g. 10,000 rpm and 16,000 rpm) and data is collected every 2-4 hours and for up to 48 hours.
Samples are prepared by the users and application specialist will run the experiment.
Inquire
AUC - Sedimentation velocity (per run)
enable the determination of the sedimentation coefficient (including distribution of species in a heterogeneous sample), diffusion coefficient, frictional/axial ratio (i.e. shape), and rough estimates of molecular weight.
Experiments generally require 380μl of sample and 400μl of reference (i.e. buffer blank)
experiments are conducted at high angular velocities (e.g. 40,000 rpm) and data is collected at short time points (e.g. every 3-10 mins) for up to 6 hours.
Samples are prepared by the users and application specialist will run the experiment.
Inquire
Maybridge F19 Fragment Library
Maybridge F19 Fragment library includes 388 fluorinated compounds to accelerate structure-based lead identification.
This library was purchased with the funds supplied from the Australian Cancer Research Foundation (ACRF) for the development of the ACRF Facility for Innovative Cancer Drug Discovery at Bio21.
Melbourne Protein Characterisation at Bio21 is part of ACRF Facility for Innovative Cancer Drug Discovery.
Inquire
Open Eye Software
Open Eye software allows us to dock the structures of millions of commercially available compounds, one at a time, into the crystal structures and calculate how well each compound binds.
This software was purchased with the funds supplied from the Australian Cancer Research Foundation (ACRF) for the development of the ACRF Facility for Innovative Cancer Drug Discovery at Bio21.
Melbourne Protein Characterisation at Bio21 is part of ACRF Facility for Innovative Cancer Drug Discovery.
Inquire
Peptide Synthesis (per residue)
The peptide synthesis is performed by the platform specialist as requested.
Inquire
Protein Crystallisation - Initial Screening
This service includeds the drop setting and imaging of one crystallisation plate per booking.
This service requires a 30 min slot that has already been booked under the "Schedule Equipment" tab.
Enable accurate determination of the native molecular weight(s) of a sample, estimation of the binding affinity (i.e. KD) of self- and hetero-associating systems and stoichiometry.
Experiments generally require 100μl of sample and 120μl of reference (i.e. buffer blank)
Experiments are conducted at multiple low angular velocities (e.g. 10,000 rpm and 16,000 rpm) and data is collected every 2-4 hours and for up to 48 hours.
Samples are prepared by the users and application specialist will run the experiment.
Inquire
AUC - Sedimentation velocity (per run)
enable the determination of the sedimentation coefficient (including distribution of species in a heterogeneous sample), diffusion coefficient, frictional/axial ratio (i.e. shape), and rough estimates of molecular weight.
Experiments generally require 380μl of sample and 400μl of reference (i.e. buffer blank)
experiments are conducted at high angular velocities (e.g. 40,000 rpm) and data is collected at short time points (e.g. every 3-10 mins) for up to 6 hours.
Samples are prepared by the users and application specialist will run the experiment.
Inquire
Peptide Synthesis (per residue)
The peptide synthesis is performed by the platform specialist as requested.
Maybridge F19 Fragment library includes 388 fluorinated compounds to accelerate structure-based lead identification.
This library was purchased with the funds supplied from the Australian Cancer Research Foundation (ACRF) for the development of the ACRF Facility for Innovative Cancer Drug Discovery at Bio21.
Melbourne Protein Characterisation at Bio21 is part of ACRF Facility for Innovative Cancer Drug Discovery.
Inquire
Open Eye Software
Open Eye software allows us to dock the structures of millions of commercially available compounds, one at a time, into the crystal structures and calculate how well each compound binds.
This software was purchased with the funds supplied from the Australian Cancer Research Foundation (ACRF) for the development of the ACRF Facility for Innovative Cancer Drug Discovery at Bio21.
Melbourne Protein Characterisation at Bio21 is part of ACRF Facility for Innovative Cancer Drug Discovery.
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